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VDI 4257 Blatt 2 Bioaerosols and biological agents - Emission measurement - Sampling of bioaerosols and separation in liquids

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German title
Bioaerosole und biologische Agenzien - Messen von Emissionen - Probenahme von Bioaerosolen und Abscheidung in Flüssigkeiten
Publication date
Engl. VDI/DIN-Kommission Reinhaltung der Luft (KRdL) - Normenausschuss
Technical Division Environmental Measurement Technologies
Related manuals
Number of pages
Available in
German, English

The guideline specifies the requirements for active sampling of bioaerosols from exhaust air flowing through a defined cross-section. It defines general principles that have to be taken into account during an emission measurement of bioaerosols. Sampling of bioaerosols takes place in consideration of the isokinetic sampling according to VDI 2066 Part 1 under application of an emission impinger. Separation of microorganisms in the impinger designed for a sample volume flow of 1 m3/h to 1,8 m3/h, or 16 l/min to 30 l/min, respectively, and has been tested with regard to various microorganisms within broad concentration ranges. This method is accepted by convention as reference method for determination of total emissions under application of an out stack configuration according to VDI 2066 Part 1. Sampling according to this guideline is a precondition for the extraction of a sample for a subsequent detection of moulds according to guideline VDI 4253 Part 2 and of bacteria according to VDI 4253 Part 3, and for determination of the total cell count according to VDI 4253 Part 4.

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