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VDI 4330 Blatt 5 Monitoring the effects of genetically modified organisms (GMOs) - Guidelines for the collection and preparation of plant samples for molecular biological analysis

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German title
Monitoring der Wirkungen gentechnisch veränderter Organismen (GVO) - Leitfaden zur Entnahme und Aufarbeitung von Pflanzenproben für die molekularbiologische Analytik
Publication date
2015-11
Publisher
Engl. VDI-Gesellschaft Technologies of Life Sciences
Related manuals
Number of pages
15
Available in
German, English
Abstract

The standard describes and standardises procedures for collecting plant samples in the field and for extracting DNA (deoxyribonucleic acid) from these samples for further molecular-biological investigations, such as polymerase chain reaction (PCR). The aim is to detect the presence or absence of GMOs. The standard gives precise instructions for handling plant material, from collecting the samples right up to storing them in the laboratory. It describes two standardised procedures for extracting nucleic acid from plant material; the CTAB method and extraction using silicium dioxide. The standard is suitable for GMO monitoring and various other research investigations.

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