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VDI 4253 Blatt 4

Bioaerosols and biological agents - Determination of total cell count by fluorescence analysis after staining with DAPI

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German title

Bioaerosole und biologische Agenzien - Bestimmung der Gesamtzellzahl mittels Fluoreszenzanalyse nach Anfärbung mit DAPI

Publication date
Engl. VDI/DIN-Kommission Reinhaltung der Luft (KRdL) - Normenausschuss
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Number of pages
Available in
German, English

The guideline describes an evaluation method for quantification of the total cell count of microorganisms by microscopic counting after staining of DNA-containing structures. It is a cultivation-independent method for analysis of ambient air samples, emission samples and indoor air samples in the course of which living and dead microorganisms are detected. The use of this analytical method is particularly advisable if microorganisms are subject to hostile environmental impacts on their way from the emission source to the sampling location. This guideline is aimed at a concentration range starting from 10<(hoch)5> GZZ/m<(hoch)3> for sampling with impingers at an air sampling volume of 0,5 m<(hoch)3>. For lower expected total cell counts higher air sampling volumes are necessary which can be obtained by means of filtration methods with polycarbonate filters.

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